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Factors affecting cryoprotectability of cultured rice (Oryza sativa L.) cells: cell wall and cell-aggregate size.

Author(s) : WATANABE K., KAWAI F., KANAMORI M.

Type of article: Article

Summary

Viability of protoplasts isolated from cells of low cryoprotectable cell lines was higher than that of intact cells, demonstrating that removal of the cell-wall fraction increased freezing tolerance. On the opposite, viability of protoplasts from cells of high cryoprotectable lines was equivalent to that of intact cells. In low cryoprotectable cell lines, large and intermediate cell aggregates displayed higher viability rate after cryopreservation than small cell aggregates, whereas cell-aggregate size did not affect viability of high cryoprotectable cells.

Details

  • Original title: Factors affecting cryoprotectability of cultured rice (Oryza sativa L.) cells: cell wall and cell-aggregate size.
  • Record ID : 1996-1619
  • Languages: English
  • Source: Cryo-Letters - vol. 16 - n. 3
  • Publication date: 1995/05
  • Document available for consultation in the library of the IIR headquarters only.

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