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Ultrastructural study on mechanism of increased freezing tolerance due to extracellular glucose in cabbage leaf cells.

Author(s) : JITSUYAMA Y., SUZUKI T., HARADA T., FUJIKAWA S.

Type of article: Article

Summary

Glucose caused increased tolerance to equilibrium slow freezing of cabbage leaf tissues, with a decrease in the temperature for the loss of 50% of electrolytes from -5 deg C, in untreated controls, to -17 deg C in samples that had been pre-incubated with a 1 M solution of glucose for 10 minutes. The brief incubation with glucose induced plasmolysis. The increased freezing tolerance of plasmolyzed cells was related to a reduction in the frequency of occurence of ultrastructural changes in plasma membranes that were produced by the close apposition of membranes. It is suggested that reduction in the extent of the close apposition of membranes might be due principally to the mitigation of severe deformation of cell walls during freezing by the presence of glucose in periprotoplasmic spaces.

Details

  • Original title: Ultrastructural study on mechanism of increased freezing tolerance due to extracellular glucose in cabbage leaf cells.
  • Record ID : 1998-0333
  • Languages: English
  • Source: Cryo-Letters - vol. 18 - n. 1
  • Publication date: 1997/01
  • Document available for consultation in the library of the IIR headquarters only.

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