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FATE OF LISTERIA MONOCYTOGENES DURING FREEZING AND FROZEN STORAGE.

Author(s) : KEST S. E. el-, YOUSEF A. E., MARTH E. H.

Type of article: Article

Summary

A CELL SUSPENSION OF LISTERIA MONOCYTOGENES WAS FROZEN 30 MIN AT -18 DEG C, OR 10 MIN IN LIQUID NITROGEN (LN) AT -198 DEG C. SOLIDIFICATION REQUIRED 15 MIN AT -18 DEG C AND ABOUT 1 MIN AT -198 DEG C. FREEZING AND STORAGE 1 MONTH IN PHOSPHATE BUFFER (PB) AT -18 DEG C CAUSED 87 % DEATH AND 79 % THESE WERE 54 AND 45 %, RESPECTIVELY, FOR CELLS IN TRYPTOSE BROTH (TB) AT -18 DEG C. FREEZING AND STORAGE 1 MONTH IN LN CAUSED NO DEATH OR INJURY OF CELLS SUSPENDED IN PB, WHEREAS SOME INJURY AND DEATH OCCURRED IN TB. FREEZING AT -198 DEG C FOLLOWED BY STORAGE 1 MONTH AT -18 DEG C RESULTED IN 60 % DEATH AND 36 % INJURY IN PB, AND 61 AND 44.2 %, IN TB. REPEATED FREEZING AND THAWING CAUSED MORE DEATH/INJURY THAN DID A SINGLE FREEZE-THAW CYCLE.

Details

  • Original title: FATE OF LISTERIA MONOCYTOGENES DURING FREEZING AND FROZEN STORAGE.
  • Record ID : 1992-1936
  • Languages: English
  • Source: Ital. J. Food Sci. - vol. 56 - n. 4
  • Publication date: 1991
  • Document available for consultation in the library of the IIR headquarters only.

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