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FREEZING INJURY IN SACCHAROMYCES CEREVISIAE: THE EFFECT OF GROWTH CONDITIONS.

Author(s) : MORRIS G. J., COULSON G. E., CLARKE K. J.

Type of article: Article

Summary

THE CHANGES IN MORPHOLOGY OF SACCHAROMYCES CEREVISIAE DURING FREEZING AND THAWING WERE OBSERVED CRYOMICROSCOPICALLY. WITH CELLS FROM A LATE EXPONENTIAL PHASE CULTURE AT 297 K (24 DEG C) THERE WAS A CORRELATION BETWEEN THE MORPHOLOGY DURING FREEZING AND VIABILITY ON THAWING. AT THE OPTIMUM RATES OF COOLING FOR VIABILITY (3 TO 10 K/MIN), CELLS SHRINK EXTENSIVELY DURING FREEZING. AT GREATER RATES OF COOLING THE REDUCTION IN VIABILITY WAS ASSOCIATED WITH THE FORMATION OF INTRACELLULAR ICE. AT SUBOPTIMAL RATES OF COOLING, CELLS WERE OSMOTICALLY UNRESPONSIVE, INDICATING DAMAGE TO THE CELL ENVELOPE. CELLS OBTAINED FROM THE LATE EXPONENTIAL PHASE OF CULTURE AT BOTH HIGHER AND LOWER TEMPERATURES WERE MORE RESISTANT TO FREEZING INJURY.

Details

  • Original title: FREEZING INJURY IN SACCHAROMYCES CEREVISIAE: THE EFFECT OF GROWTH CONDITIONS.
  • Record ID : 1989-2070
  • Languages: English
  • Source: Cryobiology - vol. 25 - n. 5
  • Publication date: 1988

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