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INTERACTIONS OF COOLING RATE, WARMING RATE, GLYCEROL CONCENTRATION, AND DILUTION PROCEDURE ON THE VIABILITY OF FROZEN-THAWED HUMAN GRANULOCYTES.

Author(s) : FRIM J., MAZUR P.

Type of article: Article

Summary

A PREREQUISITE FOR THE SUCCESSFUL FREEZING OF MAMMALIAN CELLS IS THE ABILITY OF THE CELL TO TOLERATE CRYOPROTECTIVE LEVELS OF ADDITIVE. THE RESPONSE OF HUMAN GRANULOCYTES TO FREEZING TO 77 K (-196 DEG C) AND THAWING IN 1 OR 2 M GLYCEROL WAS NOT UNUSUAL. THE OPTIMUM COOLING RATE WAS 1-3 K/MIN, AND COOLING AT 10 K/MIN OR FASTER WAS ESPECIALLY DELETERIOUS IF WARMING WAS SLOW (1 K/MIN) RATHER THAN RAPID (188 K/MIN). THE FDA ASSAY SHOWED THAT SOME 75% OF THE CELLS SURVIVED FREEZING AND THAWING AT OPTIMUM RATES IN 1 OR 2 M GLYCEROL ; AND SOME 50-60% REMAINED VIABLE AFTER THE GLYCEROL HAD BEEN REMOVED, PROVIDED THAT THE CELLS REMAINED AT 273 K (0 DEG C).

Details

  • Original title: INTERACTIONS OF COOLING RATE, WARMING RATE, GLYCEROL CONCENTRATION, AND DILUTION PROCEDURE ON THE VIABILITY OF FROZEN-THAWED HUMAN GRANULOCYTES.
  • Record ID : 1984-2119
  • Languages: English
  • Source: Cryobiology - vol. 20 - n. 6
  • Publication date: 1983

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