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HPLC FORMALDEHYDE RESEARCH IN FROZEN CRUSTACEANS.

[In Italian. / En italien.]

Author(s) : RENON P., BIONDI P. A., MALANDRA R.

Type of article: Article

Summary

A HPLC METHOD OF DETERMINING FORMALDEHYDE AS A FRESH-NESS INDEX OF FROZEN CRUSTACEANS IS REPORTED. FORMALDEHYDE IS COLLECTED QUANTITATIVELY BY STEAM DISTILLATION OF THE SAMPLE, ADDED TO BENZALDEHYDE AS AN INTERNAL STANDARD, THEN CLEANED UP AND DERIVED WITH 2.4 DINITROPHENYLHYDRAZINE (DNPH) ON A STRONG CATION EXCHANGE COLUMN. THE DNPH DERIVATIVES ELUTED WITH ACETONITRILE (MECN) ARE INJECTED INTO HPLC EQUIPMENT USING A CHROMOSPHERE C8 3 MICROMETER (155 X 4 MM) COLUMN AND SEPARATED WITH A MECN-H2 O (3:2) MOBILE PHASE AT A 0.6 ML/MIN FLUX WITH UV DETECTION AT 340 NM. FORMALDEHYDE IS QUANTIFIED BY THE INTERNAL STANDARD CALIBRATION METHOD. THE METHOD IS COMPARED WITH THE STANDARD SPECTROPHOTOMETRC ETD. THE LIMIT OF DETECTION IS OF 0.75 MICROGRAM PER MILLILITRE WITH A RECOVERY OF 95-98% AND RELIABILITY OF 5%. A. P.

Details

  • Original title: [In Italian. / En italien.]
  • Record ID : 1990-1505
  • Languages: Italian
  • Source: Rev. Ferment. Ind. aliment. - vol. 28 - n. 275
  • Publication date: 1989
  • Document available for consultation in the library of the IIR headquarters only.

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