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Impact of high pressure freezing on DH5 alpha Escherichia coli and red blood cells.

Author(s) : SUPPES G. J., EGAN S., CASILLAN A. J., et al.

Type of article: Article

Summary

The impact of high pressure and freezing on survivability of Escherichia coli and human red blood cells was evaluated to determine the utility of high-pressure transitions for preserving living cells. Based on microscopy and survivability, high pressures did not directly impact physical damage to living cells. E. coli studies showed that increased cell death is due to indirect phenomena with decreasing survivability at increasingly high pressures and exposure times. Pressurization rates up to 1.4 kbar/min had negligible effects relative to exposures of >5 min at high pressures. Both glycine and control of pH near 7.0 were successful in reducing the adverse impacts of high pressure. Survivability increased from <1% at 5 min exposure to 2.1 kbar of pressure to typical values >20%. The combination of glycine and the buffer salt led to even further improvements in survivability. Pressure changes were used to traverse temperature and pressures consistent with Ice I and Ice III phase boundaries of pure water.

Details

  • Original title: Impact of high pressure freezing on DH5 alpha Escherichia coli and red blood cells.
  • Record ID : 2004-1766
  • Languages: English
  • Source: Cryobiology - vol. 47 - n. 2
  • Publication date: 2003/10

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