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Low- and high-temperature vitrification of living cells: mechanisms and applications.

Author(s) : KATKOV I. I.

Summary

Cells held in a liquid milieu undergo processes that cause the progressive loss of viability. To prevent such degradation, cells need to be placed in conditions that essentially stop all chemical reactions for the duration of the time of storage. Because intracellular ice formation is lethal to most eukaryotic cells, stable storage of viable cells can be achieved only if intracellular vitrification without ice formation has occurred. That has been done by several methods, including equilibrium (slow) freezing, lyophilization (freeze-drying), and ice-free vitrification at subzero temperatures at moderate-to high cooling rates in the presence of cryoprotectants ("conventional" vitrification). In this mini-review, the author discusses mechanisms of vitrification, and specific aspects, advantages, and pitfalls of the different approaches. Particular emphasis is put on novel methods of cell preservation, such as cryoprotectant-free vitrification of sperm and high temperature vitrification by air/vacuum drying.

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Pages: 2004-1

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Details

  • Original title: Low- and high-temperature vitrification of living cells: mechanisms and applications.
  • Record ID : 2005-2264
  • Languages: English
  • Source: Cryogenics 2004. Proceedings of the 8th Cryogenics Conference
  • Publication date: 2004/04/27

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