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Osmotic effects on ram and human sperm membranes in relation to thawing injury.

Author(s) : CURRY M. R., WATSON P. F.

Type of article: Article

Summary

Human spermatozoa exhibited a precipitate decline in survival at osmolalities below 90 mOsm caused by cells swelling beyond their maximum volume-to-surface area ratio and lysing. Ram spermatozoa exhibited a progressive decline in cell survival at relatively small hyposmotic stresses before exceeding their maximum volume-to-surface area ratio; this prelytic cell loss could be prevented by decreasing the osmolality in a series of 25-mOsm steps. Repeated hyposmotic stress experiments indicated that cells sensitive to prelytic damage constitute a discrete subpopulation within the ram ejaculate. Spermatozoa of both species were apparently resistant to hyperosmotic stresses: human up to 2.5 Osm and ram up to 1 Osm. However, ram spermatozoa suffered an almost complete and irreversible loss of motility above 600 mOsm. Spermatozoa of both species exposed to hyperosmotic stress and returned to isosmotic conditions exhibited significant cell damage.

Details

  • Original title: Osmotic effects on ram and human sperm membranes in relation to thawing injury.
  • Record ID : 1995-1219
  • Languages: English
  • Source: Cryobiology - vol. 31 - n. 1
  • Publication date: 1994/02

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