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PERMEABILITY CHANGES IN YEAST AFTER FREEZE-THAW DAMAGE ; COMPARISON TO REPRODUCTIVE SURVIVAL.

Author(s) : LEPOCK J. R., KEITH A. D., KRUUV J.

Type of article: Article

Summary

SURVIVAL OF YEAST CELLS AFTER A FREEZE-THAW CYCLE WAS STUDIED IN THREE WAYS: 1. ABILITY TO FORM COLONIES, 2. IMPERMEABILITY TO NICKEL 2+ AND 3. IMPERMEABILITY TO METHYLENE BLUE. SURVIVAL WAS MEASURED AS A FUNCTION OF COOLING RATE BOTH IN THE PRESENCE AND ABSENCE OF DMSO. IMPERMEABILITY TO NICKEL 2+ WAS MEASURED BY AN ELECTRON SPIN RESONANCE TECHNIQUE. THERE WAS GOOD CORRELATION BETWEEN SURVIVAL AS MEASURED BY THIS TECHNIQUE AND THAT ASSESSED BY COLONY FORMATION. SURVIVAL AS ESTIMATED BY METHYLENE BLUE PERMEABILITY INVARIABLY GAVE RESULTS 10-20% HIGHER NEAR THE COOLING RATE OPTIMUM THAN THE OTHER TWO TECHNIQUES.

Details

  • Original title: PERMEABILITY CHANGES IN YEAST AFTER FREEZE-THAW DAMAGE ; COMPARISON TO REPRODUCTIVE SURVIVAL.
  • Record ID : 1985-1288
  • Languages: English
  • Source: Cryo-Letters - vol. 5 - n. 4
  • Publication date: 1984
  • Document available for consultation in the library of the IIR headquarters only.

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