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Enhanced survival of yeast expressing an antifreeze gene analogue after freezing.

Author(s) : MCKOWN R. L., WARREN G. J.

Type of article: Article

Summary

Yeast suffers from freezing. This is probably due to the recrystallization of intracellular ice. In the blood of certain polar fishes, some antifreeze proteins are found. Feasibility of protecting rapidly cooled yeast cells from ice recrystallization was studied, by expressing "in vivo", an antifreeze analogue gene. Processus is described. Treated yeast (Saccharomyces cerevisiae) then produces a new protein which can inhibit the recrystallization "in vitro". Such yeast cells show a twofold increase in survival after rapid freezing.

Details

  • Original title: Enhanced survival of yeast expressing an antifreeze gene analogue after freezing.
  • Record ID : 1992-3061
  • Languages: English
  • Source: Cryobiology - vol. 28 - n. 5
  • Publication date: 1991/10

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