Production of breathable vesicles containing live Legionella pneumophila cells by two Acanthamoeba spp.

Author(s) : BERK S. G., TING R. S., TURNER G. W., ASHBURN R. J.

Type of article: Article

Summary

Two Acanthamoeba species, fed at three temperatures, expelled vesicles containing living Legionella pneumophila cells. Vesicles ranged from 2.1 to 6.4 micrometres in diameter and theoretically could contain several hundred bacteria. Viable L. pneumophila cells were observed within vesicles which had been exposed to two cooling tower biocides for 24 hours. Clusters of bacteria in vesicles were not dispersed by freeze-thawing and sonication. Such vesicles may be the agents for the transmission of legionellosis associated with cooling towers, and the risk may be underestimated by plate count methods.

Details

  • Original title: Production of breathable vesicles containing live Legionella pneumophila cells by two Acanthamoeba spp.
  • Record ID : 1999-0813
  • Languages: English
  • Source: Appl. environ. Microbiol. - vol. 64 - n. 1
  • Publication date: 1998/01

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