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COLD SHOCK INJURY IN TETRAHYMENA PYRIFORMIS.

Author(s) : MORRIS G. J., COULSON G. E., CLARKE A.

Type of article: Article

Summary

CELLULAR VIABILITY WAS FOUND TO DEPEND ON THE TIME-TEMPERATURE AND THE RATE OF COOLING. DURING COOLING TO 265.5 K (-7.5 DEG C), IN THE ABSENCE OF ICE, AN OPTIMAL RATE OF COOLING OF 2.5 K/MIN WAS OBSERVED. FOLLOWING ACCLIMATION AT 283 K (10 DEG C) THE VIABILITY FOLLOWING RAPID COOLING WAS SIGNIFICANTLY DIFFERENT FROM THAT OF CULTURES MAINTAINED AT 293 K (20 DEG C). ANALYSIS OF THE PHOSPHOLIPID FATTY ACIDS FROM CELLS GROWN AT 283 K DEMONSTRATED THAT, THERE WAS AN INCREASE IN THE AVERAGE DEGREE OF FATTY ACYL UNSATURATION. COLD-SHOCK INJURY IS ASSOCIATED WITH MEMBRANE THERMOTROPIC EVENTS WHICH ARE DETERMINED BY TEMPERATURE PER SE, WHEREAS VIABILITY IS A FUNCTION OF THE RATE OF COOLING. A HYPOTHESIS OF INJURY IS PRESENTED IN WHICH THE PRESENCE OF GEL-PHASE LIPID WITHIN THE MEMBRANE IS NOT THE CRITICAL EVENT, BUT IT IS THE PATTERN OF NUCLEATION WITHIN THE MEMBRANE WHICH ULTIMATELY DETERMINES THE EXTENT OF CELLULAR INJURY.

Details

  • Original title: COLD SHOCK INJURY IN TETRAHYMENA PYRIFORMIS.
  • Record ID : 1985-2227
  • Languages: English
  • Source: Cryobiology - vol. 21 - n. 6
  • Publication date: 1984

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