SURVIVAL AT LOW TEMPERATURES OF SHOOT-TIPS FROM CULTIVARS OF -SOLANUM TUBEROSUM, GROUP TUBEROSUM.

Author(s) : TOWILL L. E.

Type of article: Article

Summary

USING 10% DMSO AS A CRYOPROTECTANT, ISOLATED SHOOT-TIPS FROM CULTIVARS OF SOLANUM TUBEROSUM GROUP TUBEROSUM SURVIVED AND RETAINED HIGH SHOOT REGENERATING CAPACITY WHEN SLOWLY COOLED TO 253K (-20 DEGC). BELOW 253K REGENERATION WAS VARIABLE. ISOLATED SHOOT-TIPS SURVIVED A COOLING PROCEDURE WITH SLOW COOLING TO 243 OR 233K (-30 OR -40 DEGC) FOLLOWED BY IMMERSION IN LIQUID NITROGEN; HOWEVER, THE PERCENTAGE OF TIPS SURVIVING WAS LOWER. THESE TIPS USUALLY FORMED CALLUS BUT OCCASIONALLY REGENERATED A SHOOT.

Details

  • Original title: SURVIVAL AT LOW TEMPERATURES OF SHOOT-TIPS FROM CULTIVARS OF -SOLANUM TUBEROSUM, GROUP TUBEROSUM.
  • Record ID : 1982-1542
  • Languages: English
  • Source: Cryo-Letters - vol. 2 - n. 12
  • Publication date: 1981
  • Document available for consultation in the library of the IIR headquarters only.

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