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SURVIVAL AT ULTRA-LOW TEMPERATURES OF SHOOT TIPS FROM SOLANUM TUBEROSUM GROUPS ANDIGENA, BHUREJA, STENOTOMUM, TUBEROSUM, AND OTHER TUBER-BEARING SOLANUM SPECIES.

Type of article: Article

Summary

THE PROCEDURE UTILIZED 10% DMSO AS THE CRYOPROTECTANT AND A COOLING RATE OF 0.2 TO 0.3 K/MIN TO 238 K (-35 DEG C) WITH SUBSEQUENT FAST COOLING TO 77 K (-196 DEG C). A HIGH PERCENTAGE OF SURVIVAL OCCURRED AS DEFINED BY THE ABILITY OF THE LOW TEMPERATURE TREATED SHOOT TIPS TO FORM CALLUS AND/OR REGENERATE A SHOOT. SURVIVAL OF S. TUBEROSUM LINES WAS ASSESSED ON A SINGLE MEDIUM, AND VARIATION AMONG LINES OCCURRED IN SHOOT REGENERATION (0-100%). THE SURVIVAL FOUND AT 77 K IN THE DIVERSE MATERIALS SUGGESTS THAT MODIFICATIONS OF THE REGENERATION PROCEDURE ARE NECESSARY FOR OPTIMAL GROWTH AND REGENERATION OF DIFFERENT CLONAL LINES.

Details

  • Original title: SURVIVAL AT ULTRA-LOW TEMPERATURES OF SHOOT TIPS FROM SOLANUM TUBEROSUM GROUPS ANDIGENA, BHUREJA, STENOTOMUM, TUBEROSUM, AND OTHER TUBER-BEARING SOLANUM SPECIES.
  • Record ID : 1985-1074
  • Languages: English
  • Source: Cryo-Letters - vol. 5 - n. 5
  • Publication date: 1984
  • Document available for consultation in the library of the IIR headquarters only.

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