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Cryopreservation of in vitro-grown gooseberry and currant meristems.

Author(s) : REED B. M., YU X.

Type of article: Article

Summary

Modifications of slow freezing, vitrification and encapsulation-dehydration were compared. Slow freezing at 0.3 or 0.5 deg C/minute following pregrowth on 5% DMSO and cryoprotection with PGD produced moderate to high survival. Vitrification in PVS2 following pregrowth on sorbitol and a 20-minute pretreatment resulted in low to moderate survival, while pregrowth on 5% DMSO improved survival for two of the three genotypes tested. Modification of the vitrification procedure with a pretreatment similar to that used in slow freezing was not effective. Encapsulation-dehydration with an 18-hour preculture with 0.75M sucrose and 3 hrs of dehydration was very effective.

Details

  • Original title: Cryopreservation of in vitro-grown gooseberry and currant meristems.
  • Record ID : 1996-1616
  • Languages: English
  • Source: Cryo-Letters - vol. 16 - n. 3
  • Publication date: 1995/05
  • Document available for consultation in the library of the IIR headquarters only.

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