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Cryopreservation of groundnut (Arachis hypogaea L) embryonic axes for germplasm conservation.

Author(s) : RUNTHALA P., JANA M. K., MOHANAN K.

Type of article: Article

Summary

Excised embryonic axes of groundnut could be successfully cryopreserved after desiccating them to 7% moisture content and with the use of 10% glycerol as cryoprotectant. A cooling rate of 2 deg C/min to a prefreezing temperature of -40 deg C was considered most effective before storage in liquid nitrogen. For regeneration, they were thawed and grown on 1/2 MS medium which resulted in high survival and regrowth. The different genotypes tested have not shown any conspicuous difference in their survival after cryopreservation.

Details

  • Original title: Cryopreservation of groundnut (Arachis hypogaea L) embryonic axes for germplasm conservation.
  • Record ID : 1994-1630
  • Languages: English
  • Source: Cryo-Letters - vol. 14 - n. 6
  • Publication date: 1993/11
  • Document available for consultation in the library of the IIR headquarters only.

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