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EFFECT OF STAGE OF DEVELOPMENT ON SURVIVAL OF MOUSE EMBRYOS FROZEN-THAWED RAPIDLY.

Author(s) : MASSIP A., ZWALMEN P. van der, LEROY F.

Type of article: Article

Summary

EMBRYOS RECOVERED ON DAY 4 OF PREGNANCY WERE CLASSIFIED INTO FOUR CATEGORIES BASED ON THEIR STAGE OF DEVELOPMENT: FROM BLASTOCYST TO MORULA. THEY WERE COOLED AT 2 K/MIN FROM 266 TO 248 K (-7 TO -25 DEG C) IN A FREEZING MEDIUM CONTAINING 1.36 M GLYCEROL AND 0.25 M SUCROSE AND THEN PLUNGED INTO LIQUID NITROGEN AND THAWED A FEW HOURS LATER IN WATER AT 293 K (20 DEG C). THE RESULTS SHOWED A CLEAR EFFECT OF THE STAGE OF DEVELOPMENT ON SURVIVAL. SURVIVAL OF EXPANDING BLASTOCYSTS AND BLASTOCYSTS WAS VERY LOW (1.4 AND 21.8%, RESPECTIVELY) COMPARED TO THAT OF EARLY BLASTOCYSTS AND COMPACTED MORULAE (69.4 AND 73.5%). AS A FURTHER TEST OF VIABILITY, 93 BLASTOCYSTS THAT HAD DEVELOPED IN CULTURE FOR 20 HR FROM 153 FROZEN-THAWED EARLY BLASTOCYSTS AND COMPACTED MORULAE (60.8%) WERE TRANSFERRED TO 8 RECIPIENT MICE. SEVEN BECAME PREGNANT, YIELDING 38/82 NORMAL LIVE YOUNG (46. 3%).

Details

  • Original title: EFFECT OF STAGE OF DEVELOPMENT ON SURVIVAL OF MOUSE EMBRYOS FROZEN-THAWED RAPIDLY.
  • Record ID : 1985-1315
  • Languages: English
  • Source: Cryobiology - vol. 21 - n. 5
  • Publication date: 1984

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