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Temperature gradients in freezing chambers of rate-controlled cooling machines.

Author(s) : KOEBE H. G., WERNER A., LANGE V., SCHILDBERG F. W.

Type of article: Article

Summary

Temperature probes with identical transmission patterns were placed at different positions within the cooling chamber of a modern cooling machine under defined conditions. Dependent on the rate of cooling, considerable differences between two-point temperature measurements were noted at various locations. On-line temperature records at a cooling rate of 10 K/min showed maximum temperature differences of 12.6 K vertically and 10.8 K horizontally at a given time during the freezing cycle. Maximum differences were recorded at absolute temperatures between 0 and -20 deg C, the crucial phase of cryopreservation of biological systems. The authors conclude that in order to define cryobiological cooling methods better, one should be aware of these differences and test each set-up individually.

Details

  • Original title: Temperature gradients in freezing chambers of rate-controlled cooling machines.
  • Record ID : 1994-1911
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 3
  • Publication date: 1993/06

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