Share

THE EFFECT OF CELL CONCENTRATION ON THE RECOVERY OF HUMAN ERYTHROCYTES AFTER FREEZING AND THAWING IN THE PRESENCE OF GLYCEROL.

Author(s) : PEGG D. E.

Type of article: Article

Summary

HUMAN ERYTHROCYTES, SUSPENDED IN 2.5 M GLYCEROL IN PHOSPHATE-BUFFERED SALINE, WERE FROZEN AT 35 K/MIN (-35C/MIN) TO BETWEEN 213 AND 203 K (-60 AND -65C) AND THAWED AT 5 K/MIN (5C/MIN). IT WAS FOUND THAT CELL CONCENTRATION HAD A MARKED -/EFFECT ON CELL RECOVERY. WHEN THE HEMATOCRIT WAS LESS THAN 20%, HEMOLYSIS WAS LESS THAN 1% BUT WHEN THE HEMATOCRIT EXCEEDED 50%, HEMOLYSIS INCREASED, REACHED 16% AT AN HEMATOCRIT 80%. POSSIBLE CAUSES OF THIS EFFECT ARE DISCUSSED, AND IT IS SUGGESTED THAT AUGMENTATION OF SOLUTION EFFECTS AND INTRACELLULAR FREEZING MAY PROVIDE A SUFFICIENT EXPLANATION. THE IMPORTANCE OF THIS CELL-PACKING EFFECT FOR ATTEMPTS TO PRESERVE WHOLE ORGANS IS DISCUSSED.

Details

  • Original title: THE EFFECT OF CELL CONCENTRATION ON THE RECOVERY OF HUMAN ERYTHROCYTES AFTER FREEZING AND THAWING IN THE PRESENCE OF GLYCEROL.
  • Record ID : 1982-0624
  • Languages: English
  • Source: Cryobiology - vol. 18 - n. 3
  • Publication date: 1981/06

Links


See other articles in this issue (4)
See the source

Indexing