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MECHANISM OF FREEZING INJURY TO ERYTHROCYTES: EFFECT OF INITIAL CELL CONCENTRATION ON THE POST-THAW HEMOLYSIS.

Author(s) : NEI T.

Type of article: Article

Summary

THE EFFECT OF CELL CONCENTRATION UPON POST-THAW HEMOLYSIS, EXAMINED MAINLY BY THE MORPHOLOGICAL OBSERVATION OF FREEZING PATTERNS IN SPECIMENS WITH OR WITHOUT CRYOPROTECTANT GLYCEROL, WAS MOST MARKED IN CONCENTRATED CELL SUSPENSIONS IN WHICH THE CELLS HAD BECOME SHRUNKEN AS A RESULT OF EXTRACELLULAR FREEZING. THE ADDITION OF GLYCEROL LESSENED THE PACKING EFFECT PROGRESSIVELY AS THE CONCENTRATION WAS INCREASED. THE RESULTS THUS OBTAINED MAY BE EXPLAINED BY ASSUMING THAT CELLS, DEFORMED IN THE FREEZING PROCESS, AND RIGID AT LOW TEMPERATURES, MIGHT UNDERGO MECHANICAL DAMAGE WHEN SUBJECTED TO COMPRESSION AND ABNORMAL CONTACT.

Details

  • Original title: MECHANISM OF FREEZING INJURY TO ERYTHROCYTES: EFFECT OF INITIAL CELL CONCENTRATION ON THE POST-THAW HEMOLYSIS.
  • Record ID : 1982-0623
  • Languages: English
  • Source: Cryobiology - vol. 18 - n. 3
  • Publication date: 1981/06

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