THE EFFECT OF COOLING RATE ON THE RELEASE OF ENCAPSULATED MARKER FROM SINGLE BILAYER DIPALMITOYLPHOSPHATIDYL CHOLINE LIPOSOMES AFTER A FREEZE/THAW PROCESS.

Type of article: Article

Summary

UNILAMELLAR DPPC/DCP-LIPOSOMES WITH VARYING CHOLESTEROL CONTENT (0/5/12.5% MOL/MOL) WERE MADE FROM LIPID/DETERGENT MICELLES USING A DIALYSIS METHOD. RELEASE OF TRAPPED FLUORESCENCE MARKER WAS MEASURED AFTER A FREEZE/THAW CYCLE APPLYING COOLING RATES BETWEEN1 AND 7,000 K/MIN. LIPOSOMES SHOWED A COOLING RATE DEPENDENT RELEASE WITH A MINIMUM AT 70 K/MIN (0% CHOLESTEROL) AND 140 K/MIN (12.5% COSR BESIDES THE SHIFT TO HIGHER COOLING RATES FOR MINIMUM RELEASE CHOLESTEROL HAD A CONCENTRATION DEPENDENT PROTECTIVE EFFECT AT LOW COOLING RATES.

Details

Original title: THE EFFECT OF COOLING RATE ON THE RELEASE OF ENCAPSULATED MARKER FROM SINGLE BILAYER DIPALMITOYLPHOSPHATIDYL CHOLINE LIPOSOMES AFTER A FREEZE/THAW PROCESS.
Record ID : 1989-2072
Languages: English
Source: Cryo-Letters - vol. 9 - n. 5
Publication date: 1988
Document available for consultation in the library of the IIR headquarters only.

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Indexing

Themes: The influence of refrigeration on cells, tissues and organs
Keywords: Cryoprotectant; Cooling rate; Cell; Liposome; Process; Cryopreservation; Freezing-thawing; Cholesterol