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Time-series of morphological change of ice crystals during freezing and thawing of biological tissues and foods (noninvasive three-dimensional fluorescence-imaging by confocal laser scanning microscope).

[In Japanese. / En japonais.]

Author(s) : ISHIGURO H.

Type of article: Article

Summary

A system for real-time three-dimensional visualization of the microscopic behaviour of ice crystals and cells (freezing pattern, morphology of ice crystals, and ice-cell interaction) during the freezing and thawing of biological tissues was developed using a confocal laser scanning microscope with a fluorescent dye. The system was applied to the following three different processes: 1) freezing and thawing of muscle tissues at a uniform temperature, 2) directional-solidifications parallel and perpendicular to the direction of muscle fibers of muscle tissues, and 3) recrystallisation of ice crystals during the slow-warming of the rapidly-frozen muscle tissues. The system and the results of the time-series morphological change of ice crystals were introduced in this article.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 2004-1979
  • Languages: Japanese
  • Source: Refrigeration - vol. 79 - n. 915
  • Publication date: 2004
  • Document available for consultation in the library of the IIR headquarters only.

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