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A COMPARATIVE STUDY OF MOUSE EMBRYO FREEZE-PRESERVATION INCLUDING THE EXAMINATION OF A THERMOELECTRIC FREEZING DEVICE.

Author(s) : SCHIEWE M. C., SCHMIDT P. M., WILDT D. E.

Type of article: Article

Summary

OVER 2,000 4-TO 8-CELL MOUSE EMBRYOS WERE RANDOMLY POOLED AND ASSIGNED TO 1 OF 12 TREATMENT GROUPS. A FACTORIAL DESIGN WAS USED TO ANALYZE 2 TYPES OF CRYOPROTECTANT/POST-THAW DILUTIONS, 2 STORAGE CONTAINERS AND 3 COOLING TREATMENTS. 2 COMMERCIAL, CONTROLLED-RATE FREEZING MACHINES WERE EXAMINED, EMPLOYING EITHER NITROGEN GAS OR THERMOELECTRIC COOLING. EMBRYOS WERE COOLED SLOWLY (0.5 K/MIN) TO 238 OR 193 K (-35 OR -80 DEG C) AND THEN COOLED RAPIDLY BY TRANSFER INTO LIQUID NITROGEN. THAWED EMBRYOS WERE CULTURED FOR 24 HOURS. CUMULATIVE RESULTS OF CONTAINER AND CRYOPROTECTANT/POST-THAW DILUTION TREATMENTS CONSISTENTLY DEMONSTRATED GREATER SURVIVAL, QUALITY AND VIABILITY RATINGS AND LOWER DEGENERATION VALUES WHEN EMBRYOS WERE FROZEN. THE HIGHEST POST-THAW SURVIVAL RATES ARE ACHIEVED WHEN EMBRYOS ARE EQUILIBRATED IN 1.5 M GLYCEROL, STORED IN GLASS AMPOULES AND SLOWLY COOLED TO 238 K BEFORE TRANSFER INTO LIQUID NITROGEN.

Details

  • Original title: A COMPARATIVE STUDY OF MOUSE EMBRYO FREEZE-PRESERVATION INCLUDING THE EXAMINATION OF A THERMOELECTRIC FREEZING DEVICE.
  • Record ID : 1989-0382
  • Languages: English
  • Source: Cryobiology - vol. 24 - n. 3
  • Publication date: 1987

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