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Cryopreservation of asparagus (Asparagus officinalis L.) embryogenic cells and subsequent plant regeneration by a simple freezing method.

Author(s) : NISHIZAWA S., SAKAI A., AMANO Y., MATSUZAWA T.

Type of article: Article

Summary

Embryogenic suspension cells of Asparagus officinalis were successfully cryopreserved (glycerol, sucrose, Linsmaier and Skoog medium), frozen at -25 deg C, prior to immersion in liquid nitrogen. After thawing, the survival rate is about 80%. This simple freezing method is a practical method for a wide range of cultured plant cells.

Details

  • Original title: Cryopreservation of asparagus (Asparagus officinalis L.) embryogenic cells and subsequent plant regeneration by a simple freezing method.
  • Record ID : 1994-1621
  • Languages: English
  • Source: Cryo-Letters - vol. 13 - n. 6
  • Publication date: 1992/11
  • Document available for consultation in the library of the IIR headquarters only.

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