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INVESTIGATION OF EXTRACELLULAR AND INTRACELLULAR CRYSTALLIZATION IN HUMAN ERYTHROCYTES USING VARIOUS COOLING REGIMENS.

[In Russian. / En russe.]

Author(s) : REPIN N. V.

Type of article: Article

Summary

THE ROLE OF EXTRACELLULAR AND INTRACELLULAR CRYSTALLIZATION IN INJURY OF HUMAN ERYTHROCYTES UNDER DIFFERENT COOLING CONDITIONS WAS STUDIED USING ELECTRON MICROSCOPY. UPTAKE OF CELLS BY EXTRACELLULAR ICECRYSTALS AND THEIR COMPRESSION WERE OBSERVED IN CELL SUSPENSIONS FROZEN WITHOUT CRYOPROTECTANTS BY ALL THE FREEZING REGIMENS USED. A MARKED DIFFERENCE IN THE CHARACTER OF INTERACTIONS BETWEEN ICE CRYSTALS AND CELLS UPON RAPID AND SLOW FREEZING IN THE PRESENCE OF CRYOPROTECTANT WAS NOTED. HETEROGENEOUS DEFORMING COMPRESSION OF CELLS BY EXTRACELLULAR ICE CRYSTALS WAS SHOWN TO BE A CAUSE OF INJURY UPON RAPID FREEZING.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1987-1605
  • Languages: Russian
  • Source: Kriobiologija - n. 3
  • Publication date: 1986
  • Document available for consultation in the library of the IIR headquarters only.

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