Share

Comparative effects of cryosolvents on tubulin association, thermal stability, and binding of microtubule-associated proteins.

Author(s) : PAJOT-AUGY E.

Type of article: Article

Summary

Tubulin association was monitored by spectrometry and sedimentation, providing the concentration in free protein, cold-depolymerizable microtubules, and cold-resistant associations. At 37 deg C, 1,2-propanediol and dimethyl sulfoxide induce a similar association level and cold stability of the assemblies. Glycerol yields a lower level of tubulin association. In addition, protein content of the various fractions obtained with microtubule-associated proteins and 30% solvent was examined by densitometry of electrophoresis gels.

Details

  • Original title: Comparative effects of cryosolvents on tubulin association, thermal stability, and binding of microtubule-associated proteins.
  • Record ID : 1994-1866
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 3
  • Publication date: 1993/06

Links


See other articles in this issue (10)
See the source

Indexing