IIR document
FREEZE-DRYING OF SACCHAROMYCES CEREVISIAE IN DIFFERENT PROTECTIVE MEDIA.
Author(s) : TSVETKOV T. D., RUMIAN N., IVANOVA S.
Summary
THE AUTHORS DISCUSS THE EFFECT OF 10 PROTECTIVE MEDIA ON THE FREEZING TO 213 K AND FREEZE-DRYING OF FIVESTEMS OF SACCHAROMYCES CEREVISIAE. THEY MADE INVESTIGATIONS INTO THE EFFECT OF SKIM MILK ADDED WITH SUGARS ON FREEZE-DRYING AND THEY OBSERVED VARIOUS RATES OF RESISTANCE TO FREEZING AND FREEZE-DRYING ACCORDING TO THE PROTECTIVE MEDIA USED. THE VIABILITY OF YEASTS IS PRESERVED AT BEST BETWEEN 81.2 AND 98.5% BY FREEZING WITH 10% GLYCERIN, 2. 5% SODIUM CHLORIDE AND WITH 5% LACTOSE AND 2.5% SODIUM CHLORIDE. AFTER FREEZE-DRYING THE AUTHORS OBSERVED A CONSIDERABLE EFFECTOF 2.5% SODIUM CHLORIDE AND 5% LACTOSE IN AQUEOUS SOLUTION OR COMBINED WITH SKIM MILK. THE SURVIVAL RATE IS 34.2%.
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Details
- Original title: FREEZE-DRYING OF SACCHAROMYCES CEREVISIAE IN DIFFERENT PROTECTIVE MEDIA.
- Record ID : 1988-1702
- Languages: English
- Source: Development in refrigeration, refrigeration for development. Proceedings of the XVIIth international Congress of Refrigeration.
- Publication date: 1987/08/24
- Document available for consultation in the library of the IIR headquarters only.
Links
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Indexing
- Themes: Freeze-drying in biology and medicine
- Keywords: Yeast; Freeze-drying; Cryoprotectant; Cell; Survival; Skimmed milk; Glycerol; Lactose
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