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Cryopreservation of dried shoot tips of mulberry winter buds and subsequent plant regeneration.

Author(s) : NIINO T., SAKAI A., YAKUWA H.

Type of article: Article

Summary

Shoot tips of mulberry winter buds were successfully cryopreserved following several preparative methods: slow prefreezing from 0 to -20 deg C in 5 deg C steps at daily intervals is more effective than 10 deg C steps at enhancing shoot tip survival after cryopreservation; excised shoot tips dehydrated to 19% expressed about 80% shoot regeneration after liquid nitrogen treatment; shoot tips trapped into alginate-coated beads containing 0.5 M sucrose were precultured on a medium supplemented with 1.0 M sucrose and then dehydrated up to about 22 to 25% (FW) followed by a plunge in LN. Approximately 70% shoot formation was achieved. Relative merits of each method are discussed.

Details

  • Original title: Cryopreservation of dried shoot tips of mulberry winter buds and subsequent plant regeneration.
  • Record ID : 1993-0260
  • Languages: English
  • Source: Cryo-Letters - vol. 13 - n. 1
  • Publication date: 1992/01
  • Document available for consultation in the library of the IIR headquarters only.

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