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CRYOPROTECTION OF RED BLOOD CELLS BY 1,3-BUTANEDIOL AND 2,3-BUTANEDIOL.

Author(s) : MEHL P., BOUTRON P.

Type of article: Article

Summary

1,3-BUTANEDIOL AND 2,3-BUTANEDIOL HAVE BEEN USED IN BUFFERED SOLUTIONS WITH 20, 30 OR 35% (W/W) ALCOHOL TO COOL ERYTHROCYTES TO 77 K (-196 DEG C) AT DIFFERENT COOLING RATES BETWEEN 0.017 AND 58 K/SEC FOLLOWED BY SLOW OR RAPID REWARMING. 1,3-BUTANEDIOL SHOWS THE SAME SHAPES OF RED BLOOD CELL SURVIVAL CURVES AS 1,2-PROPANEDIOL. THE CLASSICAL MAXIMUM OF SURVIVAL FOR INTERMEDIATE COOLING RATES AND AN INCREASE FOR THE HIGHEST COOLING RATES ARE OBSERVED WHICH SEEMS TO BE CORRELATED WITH THE GLASS-FORMING TENDENCY OF THE SOLUTION. AFTER THE FASTEST COOLING RATES, A WARMING RATE OF 83 K/SEC IS SUFFICIENT TO AVOID CELL DAMAGE, BUT A WARMING RATE OF 1.7 TO 3.3 K/SEC IS NOT.

Details

  • Original title: CRYOPROTECTION OF RED BLOOD CELLS BY 1,3-BUTANEDIOL AND 2,3-BUTANEDIOL.
  • Record ID : 1989-0349
  • Languages: English
  • Source: Cryobiology - vol. 25 - n. 1
  • Publication date: 1988

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