Share

Effect of thawing and recovery conditions on the regrowth of meristems of date palm (Phoenix dactylifera L.) after cryopreservation in liquid nitrogen.

Author(s) : BAGNIOL S., ENGELMANN F.

Type of article: Article

Summary

A thawing temperature of 40 deg C was optimal compared with 60 and 80 deg C or slow rewarming at room temperature. Recovery was improved by placing the thawed meristems in the dark for ten days and then under attenuated light on a medium containing 1 gramme/liter activated charcoal. In these conditions, up to 30% of the cryopreserved meristems could withstand freezing in liquid nitrogen. This technique was successfully applied to two other varieties of date palm.

Details

  • Original title: Effect of thawing and recovery conditions on the regrowth of meristems of date palm (Phoenix dactylifera L.) after cryopreservation in liquid nitrogen.
  • Record ID : 1993-0268
  • Languages: English
  • Source: Cryo-Letters - vol. 13 - n. 4
  • Publication date: 1992/07
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (4)
See the source

Indexing