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EFFECTS OF FREEZING ON THE STRUCTURE OF CHLOROPLAST MEMBRANES.

Author(s) : JENSEN M., OETTMEIER W.

Type of article: Article

Summary

ELECTRON SPIN RESONANCE (ESR) SPECTRA OF STEARIC ACID SPIN LABELS INCORPORATED INTO SPINACH THYLAKOIDS CAN BE USED TO MONITOR MEMBRANE CHANGES DURING FREEZING. CHANGES IN THE ESR PARAMETERS CAN BE DIRECTLY CORRELATED TO THE EXTENT OF FUNCTIONAL FREEZE DAMAGE. FREEZE-INDUCED CHANGES IN THE ESR PARAMETERS STRONGLY DEPEND ON THE OSMOTIC CONDITIONS OF THE INCUBATION MEDIUM. FREEZE-INDUCED ESR SPECTRAL CHANGES ARE DUE TO SWELLING OF THE THYLAKOIDS. DURING FREEZING, SALT AND/OR SUGAR LEAK INTO THE LUMEN. SIMULTANEOUSLY, PROTON CHANNELS ARE IRREVERSIBLY OPENED. STRUCTURAL ALTERATIONS OBTAINED UPON FREEZING ARE NOT ACCOMPANIED BY A CHANGE IN BULK FLUIDITY.

Details

  • Original title: EFFECTS OF FREEZING ON THE STRUCTURE OF CHLOROPLAST MEMBRANES.
  • Record ID : 1985-1292
  • Languages: English
  • Source: Cryobiology - vol. 21 - n. 4
  • Publication date: 1984

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