Share

LOW TEMPERATURE STORAGE OF HUMAN DIPLOID FIBROBLAST.

Author(s) : BITTLES A. H.

Type of article: Article

Summary

IT IS NOW POSSIBLE TO MAINTAIN CULTURED CELLS AT SUB-ZERO TEMPERATURES FOR EXTENSIVE PERIODS OF TIME WITHOUT DETECTABLE CHANGES IN THEIR CHROMOSOMAL STRUCTURE OR IN THEIR GROWTH CHARACTERISTICS. INTRACELLULAR ICE FORMATION IS AVOIDED BY THE EMPLOYEMENT OF SLOW FREEZING, BETWEEN 1.5.C MN-1 AND 4.5. C MN-1 WITH HUMAN DIPLOID FIBROPLASTS AND 30.C MN-1 FOR ANEUPLOID HUMAN CELLS. THE ROLE OF THE CELL MEMBRANE IN THE FREEZE-THAW DAMAGE IS DISCUSSED. TWO MAIN GROUPS OF CRYOPROTECTANTS ARE DESCRIBED: THOSE WHICH ENTER THE CELL AND ACT TO PREVENT AN INCREASE IN THE INTRACELLULAR SOLUTE CONCENTRATION BY BINDING WATER MOLECULES (GLYCEROL, DIMETHYLSULFOXIDE) AND A GROUP OF NON-PENETRATING SUBSTANCES WHOSE ROLE IS THOUGHT TO BE THE PROTECTION OF THE MEMBRANE (POLYVINYLPYRROLIDONE, HYDROXYETHYL STARCH). CULTURED HUMAN DIPLOID FIBROBLASTS HAVE BEEN SUCCESSFULLY STORED AT -196.C (77 K) FOR OVER 7 YEARS AND AN AVERAGE RECOVERY RATE OF VIABLE CELLS OF 60% TO 100% CAN BE EXPECTED. J. V.

Details

  • Original title: LOW TEMPERATURE STORAGE OF HUMAN DIPLOID FIBROBLAST.
  • Record ID : 1982-0289
  • Languages: English
  • Source: Indian J. Cryog. - vol. 4 - n. 3
  • Publication date: 1980
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (2)
See the source

Indexing