CRYOPRESERVATION OF SPINACH CHLOROPLAST MEMBRANES BY LOW-MOLECULAR-WEIGHT CARBOHYDRATES. I. EVIDENCE FOR CRYOPROTECTION BY A NONCOLLIGATIVE-TYPE MECHANISM.

Author(s) : SANTARIUS K. A., BAUER J.

Type of article: Article

Summary

IN FREEZING EXPERIMENTS WITH ISOLATED SPINACH THYLAKOIDS (SPINACIA OLERACEA L. CV MONATOL) THE CRYOPROTECTIVE EFFICIENCY OF VARIOUS LOW-MOLECULAR-WEIGHT POLYOLS WAS DETERMINED. THE ACTIVITY OF CYCLIC PHOTOPHOSPHORYLATION WAS USED AS AN ASSAY FOR THE FUNCTIONAL INTEGRITY OF THE MEMBRANES. THE RESULTS WERE COMPARED WITH THE OSMOTIC BEHAVIOUR OF THE CRYOPROTECTANTS AT HIGH CONCENTRATIONS. FROM THE DATA IT IS SUGGESTED THAT IN ADDITION TO COLLIGATIVE ACTION OF THE COMPOUNDS, A SPECIFIC NONCOLLIGATIVE MECHANISM CONTRIBUTES TO MEMBRANE PROTECTION DURING FREEZING.

Details

  • Original title: CRYOPRESERVATION OF SPINACH CHLOROPLAST MEMBRANES BY LOW-MOLECULAR-WEIGHT CARBOHYDRATES. I. EVIDENCE FOR CRYOPROTECTION BY A NONCOLLIGATIVE-TYPE MECHANISM.
  • Record ID : 1983-1995
  • Languages: English
  • Source: Cryobiology - vol. 20 - n. 1
  • Publication date: 1983

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