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Electron microscopic observation and biochemical properties of carp myosin B during frozen storage at -8 deg C with cryoprotectants.

[In Japanese. / En japonais.]

Author(s) : INOUE N., et al.

Type of article: Article

Summary

The cryoprotective effect of sorbitol (0.5 M) and monosodium glutamate (0.3 M) was examined on the freeze denaturation of carp myosin B filaments by observing the morphological changes in electron microscopy. Myosin B in the presence of 0.1 or 0.6 M of potassium chloride was stored at -8 deg C that was higher temperature that the eutectic point of potassium chloride and provided the concentrated potassium-chloride solution for causing the filamentous structure to deform. In the case of frozen storage in 0.1 M of potassium chloride, the deformation of myosin B filaments was protected with both cryoprotectants. In the case of 0.6 M of potassium chloride with monosodium glutamate, the deformation of the filaments was prevented. However, the granular matters deformed from myosin B were observed to some extent in 0.6 M of potassium-chloride with sorbitol. Morphological changes of the filaments in the electron microscopy agreed with the changes in biochemical properties of myosin B.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 1992-2867
  • Languages: Japanese
  • Source: Trans. JAR - vol. 9 - n. 1
  • Publication date: 1992
  • Document available for consultation in the library of the IIR headquarters only.

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