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FREEZE-INDUCED ELECTRICAL TRANSIENTS AND CRYOINJURY.

Author(s) : STEPONKUS P. L.

Type of article: Article

Summary

DURING THE FREEZING OF AQUEOUS SOLUTIONS, LARGE ELECTRICAL POTENTIAL DIFFERENCES BETWEEN THE LIQUID AND SOLID PHASES MAY ARISE BECAUSE OF THE PREFERENTIAL EXCLUSION OF ONE SIGN OF CHARGE FROM THE ICE (THE WORKMAN-REYNOLDS EFFECT). FREEZING PROTOPLAST SUSPENSIONS TO 263 K (-10 DEG C) AT RAPID RATES RESULTED IN POTENTIALS OF NEARLY 4 V AND SURVIVAL WAS LOW (12%). DURING SLOW COOLING TO 269 K (-4 DEG C), NO MEASURABLE POTENTIAL OCCURRED AND PROTOPLAST SURVIVAL WAS HIGH (60%). A DISCUSSION OF THE THEORY OF THE WORKMAN-REYNOLDS EFFECT AND ITS POSSIBLE CONTRIBUTION TO MEMBRANE DISRUPTION AND INTRACELLULAR ICE FORMATION IS PRESENTED.

Details

  • Original title: FREEZE-INDUCED ELECTRICAL TRANSIENTS AND CRYOINJURY.
  • Record ID : 1985-1290
  • Languages: English
  • Source: Cryo-Letters - vol. 5 - n. 5
  • Publication date: 1984
  • Document available for consultation in the library of the IIR headquarters only.

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