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FUNCTIONAL TESTING OF HEPATOCYTES FOLLOWING THEIR RECOVERY FROM CRYOPRESERVATION.

Author(s) : INNES G. K., FULLER B. J., HOBBS K. E. F.

Type of article: Article

Summary

THE AUTHORS HAVE INVESTIGATED HEPATOCYTE ATTACHMENT DURING TISSUE CULTURE AND CELLULAR DENSITY IN ORDER TO ASSESS FUNCTION AND COMPARED THEM WITH 2 CLASSICAL DYE EXPOSURE TESTS. THEY CONCLUDE THAT CRYOPRESERVATION PRODUCES A POPULATION OF CELLS WHICH ARE NOT METABOLICALLY IDENTICAL TO A POPULATION OF FRESH-LY PREPARED HEPATOCYTES EVEN THOUGH THEY APPEAR TO HAVE THE SAME BUOYANT DENSITY AND DYE-EXCLUDING CAPABILITIES. THEY SUGGEST THAT THERE IS NO SINGLE DEFINITIVE TEST FOR THE SUCCESSFUL CRYOPRESERVATION OF THIS TYPE OF CELL AND THAT ANY FUNCTIONAL TEST PERFORMED IS ONLY RELEVANT TO THAT SPECIFIC HEPATOCELLULAR PROCESS UNDER INVESTIGATION.

Details

  • Original title: FUNCTIONAL TESTING OF HEPATOCYTES FOLLOWING THEIR RECOVERY FROM CRYOPRESERVATION.
  • Record ID : 1989-0355
  • Languages: English
  • Source: Cryobiology - vol. 25 - n. 1
  • Publication date: 1988

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