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LIPASE RELEASE FROM LYSOSOMES OF RAINBOW TROUT (SALMO GAIRDNERII) MUSCLE SUBJECTED TO LOW TEMPERATURES.

Author(s) : GEROMEL E. J., MONTGOMERY M. W.

Type of article: Article

Summary

LYSOSOMAL LIPASES OF THE DARK LATERAL LINE MUSCLE OF RAINBOW TROUT) HAD PH OPTIMA OF 4.2-4.5 AND 7.8. THE ACID LIPASE WAS CHOSEN FOR FURTHER STUDY. PRESENCE OF TRITON-X-100 IN THE REACTION MIXTURE ENHANCED THE ACTIVITY OF THE ACID LIPASE. FASTING THE FISH FOR 24 AND 72 HR PRIOR TO SACRIFICE RESULTED IN HIGHER ACID LIPASE ACTIVITIES. LYSOSOMAL PREPARATIONS CONTAINED 60% OF THE ACID LIPASE OF THE DARK LATERAL LINE MUSCLE. STORAGE ON ICE AND FAST AND INTERMEDIATE RATES OF FREEZING DID NOT CAUSE THE RELEASE OF ACID LIPASE FROM THE LYSOSOMES. HOWEVER, SLOW FREEZING AND FLUCTUATION OF TEMPERATURES [261 TO 238 K (-12 TO -35 C) FOR 96 HR] OF THE FROZEN FILLETS RESULTED IN APPRECIABLE RELEASE OF THE ACID LIPASE FROM LYSOSOMES.

Details

  • Original title: LIPASE RELEASE FROM LYSOSOMES OF RAINBOW TROUT (SALMO GAIRDNERII) MUSCLE SUBJECTED TO LOW TEMPERATURES.
  • Record ID : 1981-0170
  • Languages: English
  • Publication date: 1980/05
  • Source: Source: J. Food Sci.
    vol. 45; n. 3; 412-415; 419; 2 fig.; 4 tabl.; ref.
  • Document available for consultation in the library of the IIR headquarters only.

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