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MEMBRANE STABILIZATION DURING FREEZING: THE ROLE OF TWO NATURAL CRYOPROTECTANTS, TREHALOSE AND PROLINE.

Author(s) : RUDOLPH A. S., CROWE J. H.

Type of article: Article

Summary

ISOLATED VESICLES OF SARCOPLASMIC RETICULUM FROM LOBSTER MUSCLE (HOMARUS AMERICANUS) WERE EMPLOYED TO STUDY CHANGES IN STRUCTURE AND FUNCTION DURING RAPID FREEZE-THAW CONDITIONS. BOTH PROLINE AND TREHALOSE WERE SHOWN TO EFFECTIVELY PRESERVE THE STRUCTURE (ASSESSED WITH FREEZE FRACTURE) AND FUNCTION (ASSESSED BY THE ABILITY OF THE MEMBRANES TO TRANSPORT CALCIUM) IN THE FROZEN VESICLES. THEY WERE FOUND TO BE MORE EFFECTIVE IN PREVENTING MEMBRANE MIXING BETWEEN SMALL UNILAMELLAR VESICLES THAN THE STANDARD PROTECTANTS, GLYCEROL AND DMSO.

Details

  • Original title: MEMBRANE STABILIZATION DURING FREEZING: THE ROLE OF TWO NATURAL CRYOPROTECTANTS, TREHALOSE AND PROLINE.
  • Record ID : 1986-1710
  • Languages: English
  • Source: Cryobiology - vol. 22 - n. 4
  • Publication date: 1985

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