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NEW BIOCHEMICAL METHODS OF DISTINGUISHING BETWEEN FRESH LIVER AND THAWED, FROZEN LIVER.

Author(s) : GOTTESMANN P., HAMM R.

Type of article: Article

Summary

THE PRINCIPLE OF THE TWO NEW METHODS IS THAT THE DAMAGE CAUSED TO THE LIVER MITOCHONDRIA BY FREEZING RELEASES THE MITOCHONDRIAL ENZYME BETA-HYDROXYACYL-COENZYME A-DEHYDROGENASE (HADH) INTO THE CELL PLASMA (PRESS JUICE) OF THE LIVER TISSUE. INCREASED HADH ACTIVITY IN THE PRESS JUICE OF THE LIVER THUS INDICATES THAT IT HAS BEEN FROZEN AND THAWED. IN ONE METHOD THE RELEASED HADH ACTIVITY IS MEASURED IN THE PRESS JUICE PHOTOMETRICALLY BY MEANS OF AN ENZYME TEST. IN THE OTHER METHOD IT IS DETERMINED BY MEANS OF A SIMPLE COLOUR REACTION (FRESH LIVER: COLOURLESS ; THAWED, FROZEN LIVER: BLUE). THE METHODS CAN BE USED ON LIVER TISSUE FROM CATTLE AND PIGS. THE RELIABILITY OF THE METHODS IS NOT IMPAIRED BY KEEPING THE LIVER CHILLED: 274 K (2 DEG C) FOR SEVEN DAYS.

Details

  • Original title: NEW BIOCHEMICAL METHODS OF DISTINGUISHING BETWEEN FRESH LIVER AND THAWED, FROZEN LIVER.
  • Record ID : 1986-0255
  • Languages: English
  • Source: Fleischwirtschaft - vol. 65 - n. 5
  • Publication date: 1985/05
  • Document available for consultation in the library of the IIR headquarters only.

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