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ON THE ROLE OF GLUTARALDEHYDE FOR CRYOPROTECTION IN FREEZE-FRACTURING OF CELLS AND TISSUES.

Author(s) : EDELMANN L., MORGENSTERN E.

Type of article: Article

Summary

COMPARISON OF FREEZE-FRACTURE REPLICAS OF GLUTARALDEHYDE-FIXED TISSUES WITH OR WITHOUT GLYCEROL TREATMENT REVEALED GOOD PRESERVATION OF ULTRASTRUCTURE. HOWEVER, IN SOME CASES INDIVIDUAL CELLULAR STRUCTURES ARE OFTEN BETTER VISUALIZED IN NON-GLYCERINATED TISSUES FOR A VARIETY OF REASONS (MICROFILAMENTS OF MUSCLE FIBRES). THIS EFFECT MAY BE CAUSED BY A HIGHER SUBLIMATION RATE OF SUCH SPECIMENS. THE GLYCOCALYX OF THE INTESTINAL EPITHELIAL CELLS WAS BETTER PRESERVED AFTER GLUTARALDEHYDE FIXATION WITHOUT GLYCEROL.

Details

  • Original title: ON THE ROLE OF GLUTARALDEHYDE FOR CRYOPROTECTION IN FREEZE-FRACTURING OF CELLS AND TISSUES.
  • Record ID : 1981-0264
  • Languages: English
  • Source: Mikroskopie - vol. 35 - n. 3-4
  • Publication date: 1979
  • Document available for consultation in the library of the IIR headquarters only.

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