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THE CRYOPRESERVATION OF TISSUES ADMITS NO APPROXIMATION.

LA CRYOCONSERVATION TISSULAIRE NE SOUFFRE PAS D'A PEU PRES.

Author(s) : DEFRANCE J.

Type of article: Article

Summary

FOR A SUITABLE FREEZING AND THAWING OF CELLS AND TISSUES, ON ONE HAND, THE ADDITION OF CRYOPROTECTANTS (GLYCEROL, DMSO) SHOULD BE USED, AT VARYING CONCENTRATIONS, ACCORDING TO THE NATURE OF THE TISSUES AND, ON THE OTHER HAND, COOLING AND WARMING RATES SHOULD BE CONTROLLED. USUALLY, COOLING TO LIQUID NITROGEN TEMPERATURE IS DONE IN TWO STEPS: SLOW COOLING TO THE FREEZING PLATEAU (THE TEMPERATURE OF WHICH VARIES WITH THE TISSUE AND THE CRYOPROTECTANT), THEN QUICK COOLING (IN ONE MINUTE) FROM THE FREEZING PLATEAU TO 77 K (-196 DEG C). IN MOST CASES, WARMING SHOULD BE QUICK. AT PRESENT, THE ELECTRONIC PROGRAMMERS OF SOME UNITS PROVIDE AN ACCURATE CONTROL OF FREEZING CONDITIONS IN TERMS OF THE TREATED TISSUE. G.G.

Details

  • Original title: LA CRYOCONSERVATION TISSULAIRE NE SOUFFRE PAS D'A PEU PRES.
  • Record ID : 1983-2376
  • Languages: French
  • Source: Quotid. Méd. - n. 2857
  • Publication date: 1983
  • Document available for consultation in the library of the IIR headquarters only.

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