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TISSUE CRYOPRESERVATION AND REWARMING STUDY ON GIANT CELL TUMOUR OF BONE.

Author(s) : SUN D.

Type of article: Article

Summary

THE TISSUE FRAGMENTS HAVE BEEN KEPT IN 10% DMSO OR 15% GLYCEROL MEDIUM AND STORED IN LIQUID NITROGEN FOR PERIODS UP TO 180 DAYS. TUMOUR CELLULAR COMPONENTS SURVIVE THE PROCESS OF SLOW FREEZING AND RAPID THAWING AS CELLULAR GROWTH IS SUCCESSFUL IN SUBSEQUENT CULTURE. ELECTRON MICROSCOPY SHOWS THAT DMSO EXCEEDS GLYCEROL IN PROTECTING THE ULTRASTRUCTURE OF THE CELLS. SLOW COOLING AND RAPID THAWING IS RECOMMENDED BECAUSE ICE-CRYSTALS FORM IN THE INTERCELLULAR SPACE, WHICH CAUSES ONLY LIGHT DAMAGE TO THE CELLS.

Details

  • Original title: TISSUE CRYOPRESERVATION AND REWARMING STUDY ON GIANT CELL TUMOUR OF BONE.
  • Record ID : 1983-2010
  • Languages: English
  • Source: Sci. sin., B - vol. 25 - n. 12
  • Publication date: 1982
  • Document available for consultation in the library of the IIR headquarters only.

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