ShareOPTIMUM WARMING RATES TO MAINTAIN GLUCOSE METABOLISM IN PORCINE SKIN CRYOPRESERVED BY SLOW COOLING.
Author(s) : MAY S. R., WAINWRIGHT J. F.
Type of article: Article
Summary
THE EXPERIMENTS ESTABLISH PRACTICAL UPPER AND LOWER LIMITS FOR POST-CRYOPRESERVATION WARMING RATES EMPLOYED TO MAINTAIN SKIN CELL METABOLISM, AND THE CELLULAR VIABILITY. SKIN COOLED AT A CONSTANT, CONTROLLED RATE OF 1 K/MIN TO A TEMPERATURE OF 173 K (-100 DEG C), AND THEN RAPIDLY TRANSFERRED TO 77 K (-196 DEG C) FOR OVERNIGHT STORAGE, COULD BE OPTIMALLY WARMED AT RATES OF 95-260 K/MIN. THE AMOUNT OF METABOLIC ACTIVITY REMAINING WAS 76-78% OF NORMAL.
Details
- Original title: OPTIMUM WARMING RATES TO MAINTAIN GLUCOSE METABOLISM IN PORCINE SKIN CRYOPRESERVED BY SLOW COOLING.
- Record ID : 1985-2684
- Languages: English
- Source: Cryobiology - vol. 22 - n. 2
- Publication date: 1985
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Reheating; Survival; Pork; Skin; Glucose; Cryopreservation
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