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PERMEATION OF DMSO INTO RYE PROTOPLASTS DURING FREEZING OF THE SUSPENDING MEDIUM.

Author(s) : MYERS S. P., STEPONKUS P. L.

Type of article: Article

Summary

RYE PROTOPLASTS (SECALE CEREALE L CV PUMA) SUSPENDED IN SALINE + 5% DMSO AND COOLED TO BETWEEN 268 AND 258 K (-5 AND -15 DEG C) DO NOT CONTRACT TO THE EXTENT PREDICTED BY A BOYLE-VAN'T HOFF RELATIONSHIP IF IT IS ASSUMED THAT DMSO DOES NOT PERMEATE DURING COOLING. THE EQUILIBRIUM VOLUME WAS A FUNCTION OF COOLING RATE AND INITIAL DMSO CONCENTRATION. IT IS PROPOSED THAT THE DEVIATION FROM THE PREDICTED BOYLE-VAN'T HOFF RELATIONSHIP RESULTS, IN PART, FROM DMSO PERMEATION DURING FREEZING OF THE SUSPENDING MEDIUM.

Details

  • Original title: PERMEATION OF DMSO INTO RYE PROTOPLASTS DURING FREEZING OF THE SUSPENDING MEDIUM.
  • Record ID : 1986-2104
  • Languages: English
  • Source: Cryo-Letters - vol. 7 - n. 1
  • Publication date: 1986
  • Document available for consultation in the library of the IIR headquarters only.

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