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The effects of variation of cryopreservation protocols on the immunogenicity of allogenic skin grafts.

Author(s) : INGHAM E., MATTHEWS J. B., KEARNEY J. N., GOWLAND G.

Type of article: Article

Summary

The study evaluated the effects of variation in the cryopreservation protocol on the viability and immunogenicity of skin using a murine model system. The following cryopreservation protocols were assessed: freezing at 1, 30, 64 and higher than 100 deg C/min in 10 and 15% DMSO and freezing at 30 deg C/min in 5 to 20% DMSO or glycerol. A cryopreservation protocol of 30 deg C/min in 15% DMSO proved optimal for murine skin allograft storage and immunomodulation. The viability of skin treated by this protocol was maintained.

Details

  • Original title: The effects of variation of cryopreservation protocols on the immunogenicity of allogenic skin grafts.
  • Record ID : 1994-1895
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 5
  • Publication date: 1993/10

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