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THE USE OF 1,2-PROPANEDIOL FOR CRYOPRESERVATION OF RECALCITRANT SEEDS: THE MODEL CASE OF ZEA MAYS IMBIBED SEEDS.

Author(s) : BOUCAUD M. T. de, CAMBECEDES J.

Type of article: Article

Summary

HYDRATED ZEA MAYS SEEDS ARE STORED AFTER RAPID FREEZING IN LIQUID NITROGEN. DIFFERENT CRYOPROTECTANTS (1,2-PROPANEDIOL, DMSO, GLYCEROL AND SUCROSE) ARE USED AND THEIR EFFECTS ARE COMPARED: 1,2-PROPANEDIOL ADDED WITH SUCROSE APPEARS THE LEAST TOXIC CRYOPROTECTANT FOR THENON-FROZEN CONTROLS AND AT A HIGH CONCENTRATION (5 M), IT IS THE MOST EFFECTIVE PROTECTANT FOR DAMAGE-FREE STORAGE OF SEEDS IN LN2.

Details

  • Original title: THE USE OF 1,2-PROPANEDIOL FOR CRYOPRESERVATION OF RECALCITRANT SEEDS: THE MODEL CASE OF ZEA MAYS IMBIBED SEEDS.
  • Record ID : 1989-0121
  • Languages: English
  • Source: Cryo-Letters - vol. 9 - n. 2
  • Publication date: 1988
  • Document available for consultation in the library of the IIR headquarters only.

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