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COMPARISON OF THE CRYOPROTECTION OF RED BLOOD CELLS BY 1,2-PROPANEDIOL AND GLYCEROL.

Author(s) : BOUTRON P., ARNAUD F.

Type of article: Article

Summary

RED BLOOD CELLS ARE COOLED IN BUFFERED SOLUTIONS CONTAINING 10, 15, 20, 30, OR 35% (W/W) 1,2-PROPANEDIOL OR GLYCEROL. CELL SURVIVAL IS MEASURED AFTER COOLING TO 77 K (-196 DEG C) AT RATES BETWEEN 1 AND 3,500 K/MIN, FOLLOWED BY REWARMING RAPIDLY, EXCEPT IN A FEW CASES. 1,2-PROPANEDIOL PROTECTS ERYTHROCYTES BETTER THAN GLYCEROL. AT HIGH COOLING RATES THE SURVIVAL PASSES THROUGH A MINIMUM. FOR THE SAME CONCENTRATIONS, THE MINIMUM OCCURS AT MUCH LOWER COOLING RATES WITH 1,2-PROPANEDIOL.

Details

  • Original title: COMPARISON OF THE CRYOPROTECTION OF RED BLOOD CELLS BY 1,2-PROPANEDIOL AND GLYCEROL.
  • Record ID : 1985-0821
  • Languages: English
  • Source: Cryobiology - vol. 21 - n. 3
  • Publication date: 1984

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