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EFFECTS OF FREEZING AND THAWING ON MAMMALIAN OOCYTE.

Author(s) : JONDET M., DOMINIQUE S., SCHOLLER R.

Type of article: Article

Summary

THE AUTHORS DEVELOPED A CRYOMICROSCOPE CONTROLLED BY DIGITAL PROGRAMMABLE EQUIPMENT WHICH PERMITS ANY COOLING RATE BETWEEN 0.1 AND 100 K/MIN. THE MAXIMUM THAWING RATE IS ABOUT 60 K/MIN. THE RESULTS SHOW THAT: (1) RAPID COOLING YIELDS A SUPERCOOLING WITH SIMULTANEOUS EXTRA-AND INTRACELLULAR CRYSTALLIZATION ; (2) SLOW COOLING WITH SEEDING AT 265 K (-8 DEG C) GIVES AN EXTRACELLULAR CRYSTALLIZATION WHICH IS ACHIEVED BY 264 K (-9 DEG C), FOLLOWED BY AN EXTRACELLULAR RECRYSTALLIZATION OCCURRING AT ALMOST 265 K ; (3) DURING CONTINUED SLOW COOLING THE OOCYTES DEHYDRATE WITHOUT ANY INTRACELLULAR FREEZING ; (4) DURING REWARMING A PARTIAL REHYDRATION OF THE CELL OCCURS.

Details

  • Original title: EFFECTS OF FREEZING AND THAWING ON MAMMALIAN OOCYTE.
  • Record ID : 1985-0819
  • Languages: English
  • Source: Cryobiology - vol. 21 - n. 2
  • Publication date: 1984

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