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FORMATION AND MELTING OF INTRACELLULAR ICE IN LYMPHOCYTES.

Author(s) : SCHEIWE M. W., KORBER C.

Type of article: Article

Summary

HUMAN LYMPHOCYTES SUSPENDED IN ISOTONIC SALINE SOLUTION WERE FROZEN ON THE COLD STAGE OF A CRYOMICROSCOPE AT CONSTANT COOLING RATES BETWEEN 10 TO 500 K/MIN AND WARMED AT CONSTANT RATES FROM 1,000 TO 4,000 K/MIN. TWO TYPES OF INTRACELLULAR ICE FORMATION OCCUR DURING COOLING, NAMELY: CRYSTALLIZATION WITH A DISTINCT ICE FRONT, AND NO ICE FRONT. THE ICE FORMATION IS SUPPOSED TO BE DEPENDENT ON THE COMPOSITION OF THE CELL INTERIOR, THE EXTENT OF INTRACELLULAR SUPERCOOLING AND THE COOLING RATE. THE VOLUME LOSS DURING FREEZING AND SWELLING DURING THAWING AND SUBSEQUENT LYSIS ARE ANALYSED.

Details

  • Original title: FORMATION AND MELTING OF INTRACELLULAR ICE IN LYMPHOCYTES.
  • Record ID : 1983-1596
  • Languages: English
  • Source: Cryo-Letters - vol. 3 - n. 5
  • Publication date: 1982
  • Document available for consultation in the library of the IIR headquarters only.

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